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Literature summary extracted from

  • Schmidt, R.J.; Zhang, Y.; Zhao, Y.; Qian, Y.W.; Wang, H.; Lin, A.; Ehsani, M.E.; Yu, X.; Wang, G.; Singh, J.; Su, E.W.; Li, S.; Konrad, R.J.; Cao, G.
    A novel splicing variant of proprotein convertase subtilisin/kexin type 9 (2008), DNA Cell Biol., 27, 183-189.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.61 cDNAs encoding full-length PCSK9 splicing variant or mutants inserted into a modified pJB02 vector with a C-terminal Flag-HIS6 or HIS6 tag, respectively. The resulting constructs used for transient transfection of HEK-293 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.61 A443T is expressed, processed, and secreted normally, and reduces cellular LDL uptake in a concentration-dependent like the wild-type Homo sapiens
3.4.21.61 additional information splicing variant of PCSK9 with an in-frame deletion of the eighth exon of 58 amino acids. Expressed in multiple tissues, including liver, small intestine, prostate, uterus, brain, and adipose tissue. Unlike wild-type PCSK9, which is secreted, the splicing variant expressed in HEK-293 cells fails to process the prosegment intracellularly and thus is not secreted into the medium. Splicing variant does not change the LDLR protein levels Homo sapiens
3.4.21.61 N425S is expressed, processed, and secreted normally, and reduces cellular LDL uptake in a concentration-dependent like the wild-type Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.61 additional information splicing variant of PCSK9 has no specific effect impacting wild-type PCSK9 function Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.61 64000
-
 mature form of wild-type PCSK9, Western blot analysis Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.61 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.61 on Ni-NTA resin Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.61 intestine
-
 Homo sapiens
-
3.4.21.61 liver highest level of expression Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
3.4.21.61 -80°C Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.21.61 PCSK9
-
 Homo sapiens
3.4.21.61 proprotein convertase subtilisin/kexin type 9
-
 Homo sapiens